2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Ibintu bisanzwe bikoreshwa mu gupima ikwirakwizwa ry'uburemere bwa molekile: insuline, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Ibikoresho n'ibikoresho
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Muri rusange, igipimo cya aside amine mu bicuruzwa bya Sustar kiri hejuru kurusha icyo mu bicuruzwa bya Zinpro.
Igice cya 8 Ingaruka zo gukoresha
Ingaruka z'amasoko atandukanye y'imyunyu ngugu ku musaruro n'ubwiza bw'amagi y'inkoko zitera mu gihe cyo gutera amagi mu mpera z'igihe cyo gutera amagi
Uburyo bwo gukora
Ikoranabuhanga rya chelation rigamije
Ikoranabuhanga ryo gukata ibyuma bivanze n'amazi
Ikoranabuhanga ryo gusukura no kumisha umuvuduko
Ikoranabuhanga ryo gushyira muri firigo no gukuraho ubushuhe
Ikoranabuhanga rigezweho ryo kugenzura ibidukikije
Umugereka A: Uburyo bwo kumenya ikwirakwizwa ry'uburemere bwa molekile bwa peptides
Kwemeza ibipimo ngenderwaho: GB/T 22492-2008
1 Ihame ry'ikizamini:
Byagenwe hakoreshejwe chromatography ya gel filtration ifite ubushobozi bwo hejuru. Ni ukuvuga, hakoreshejwe uburyo bwo gupima imyenge nk'ikiciro gihoraho, hashingiwe ku itandukaniro riri hagati y'ingano y'uburemere bwa molekile bw'ibice by'icyitegererezo kugira ngo bitandukanywe, byagaragaye ku mugozi wa peptide w'uburebure bwa ultraviolet bwo kwinjiza 220nm, hakoreshejwe porogaramu yihariye yo gutunganya amakuru kugira ngo hamenyekane ikwirakwizwa ry'uburemere bwa molekile hakoreshejwe chromatography ya gel filtration (ni ukuvuga porogaramu ya GPC), chromatograms n'amakuru yazo byaratunganyijwe, bibarwa kugira ngo hamenyekane ingano y'uburemere bwa molekile bwa peptide ya soya n'urwego rw'ikwirakwizwa.
2. Ibikoresho bitanga umusaruro
Amazi yo mu igerageza agomba kuba yujuje ibisabwa n'amazi y'inyongera muri GB/T6682, ikoreshwa ry'ibisubizo, uretse ingingo zihariye, ni nta busesenguzi.
2.1 Ibintu bigabanya ubukana birimo acetonitrile (icyiza mu buryo bwa chromatographic), aside trifluoroacetic (icyiza mu buryo bwa chromatographic),
2.2 Ibintu bisanzwe bikoreshwa mu gupima ikwirakwizwa ry'uburemere bwa molekile: insuline, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Ibikoresho n'ibikoresho
3.1 Chromatograph y'amazi ifite imikorere myiza (HPLC): ikigo cy'ikoranabuhanga cya chromatographic cyangwa integrator gifite icyuma gipima UV na porogaramu yo gutunganya amakuru ya GPC.
3.2 Igice cyo kuyungurura no gukuramo imyuka mu buryo bugendanwa.
3.3 Amafaranga asigaye mu buryo bw'ikoranabuhanga: agaciro kagabanijwe 0.000 1g.
Intambwe 4 z'imikorere
4.1 Imiterere ya kromatografiya n'igerageza ryo guhuza sisitemu (imiterere y'icyitegererezo)
- 4.1.1 Inkingi ya kromatografiya: TSKgelG2000swxl300 mm×7.8 mm (umurambararo w'imbere) cyangwa izindi nkingi za jeli z'ubwoko bumwe zifite imikorere isa ikwiriye mu kugena poroteyine na peptides.
- 4.1.2 Icyiciro cyo kugenda: Acetonitrile + amazi + aside trifluoroacetic = 20 + 80 + 0.1.
- 4.1.3 Uburebure bw'urumuri rupima: 220 nm.
- 4.1.4 Igipimo cy'amazi atemba: 0.5 mL/umunota.
- 4.1.5 Igihe cyo kumenya: iminota 30.
- 4.1.6 Ingano y'icyitegererezo cy'urushinge: 20μL.
- 4.1.7 Ubushyuhe bw'inkingi: ubushyuhe bw'icyumba.
- 4.1.8 Kugira ngo sisitemu ya chromatografiya yuzuze ibisabwa mu gupima, byagenwe ko hakurikijwe imiterere ya chromatografiya yavuzwe haruguru, imikorere ya gel chromatografiya, ni ukuvuga umubare w’amapine (N), itari munsi ya 10000 yabazwe hashingiwe ku ntera z’ibipimo bya tripeptide (Glycine-Glycine-Glycine).
- 4.2 Gukora imigozi isanzwe y'uburemere bwa molekile
- Ibisubizo bitandukanye byavuzwe haruguru bya peptide isanzwe ifite uburemere bwa 1 mg / mL byateguwe hakoreshejwe uburyo bwo guhuza imiterere y’ibice, bivangwa mu rugero runaka, hanyuma bigasukurwa binyuze mu ruhu rw’imiterere y’ibice bifite ingano ya 0.2 μm ~ 0.5 μm hanyuma bigashyirwa mu gipimo, hanyuma haboneka chromatogramu z’ibipimo. Imirongo yo gupima uburemere bwa molekile n’ibipimo byabyo byabonetse hakoreshejwe uburyo bwo gushushanya logarithm y’ubunini bwa molekile hakurikijwe igihe cyo kubigumana cyangwa hakoreshejwe umurongo ugororotse.
4.3 Icyitegererezo cy'uburyo bwo kuvura
Pima neza 10mg y'icyitegererezo mu icupa rya 10ml volumetric, ongeramo agace gato kagendanwa, ugatengure ultrasonic mu gihe cy'iminota 10, kugira ngo icyitegererezo gishonge neza kandi kivangwe, givangwe na mobile phase kugeza ku gipimo, hanyuma kinyurwe mu ruhu rw'ingirabuzimafatizo rufite imyenge ya 0.2μm ~ 0.5μm, hanyuma filtrate isesengurwe hakurikijwe imiterere ya chromatographic muri A.4.1.
- 5. Kubara ikwirakwizwa ry'uburemere bwa molekile
- Nyuma yo gusesengura igisubizo cy'icyitegererezo cyateguwe muri 4.3 hakurikijwe imiterere ya kromatografiya ya 4.1, uburemere bwa molekile y'icyitegererezo n'urwego rwacyo rukwirakwizwa bishobora kuboneka hakoreshejwe uburyo bwa kromatografiya bw'icyitegererezo mu buryo bwa calibration 4.2 na porogaramu ya GPC yo gutunganya amakuru. Ikwirakwizwa ry'ubunini bwa molekile y'ama peptide atandukanye rishobora kubarwa hakoreshejwe uburyo bwo kugena ahantu hanini, hakurikijwe formula: X=A/A igiteranyo ×100
- Muri formula: X - Igice cy'uburemere bwa peptide y'uburemere bwa molekile muri peptide yose mu gipimo, %;
- A - Agace k'impinga ya peptide y'umubare wa molekile;
- Igiteranyo A - igiteranyo cy'ubuso bw'impinga ya buri peptide y'ubunini bwa molekile, ibarwa kugeza ahantu hamwe ho kugabanya.
- 6 Gusubiramo
- Itandukaniro rigaragara hagati y’ibizamini bibiri byigenga byabonetse mu buryo bwo gusubiramo ntibigomba kurenga 15% by’ikigereranyo cy’imibare cy’ibyavuye mu bizamini byombi.
- Umugereka B: Uburyo bwo kumenya aside amine zidafite amino
- Kwemeza ibipimo ngenderwaho: Q/320205 KAVN05-2016
- 1.2 Ibikoresho bitanga umusaruro n'ibikoresho
- Aside acetiki y'ibara rya glacial: isukuye mu buryo bw'isesengura
- Aside ya perchloric: 0.0500 mol/L
- Ikimenyetso: Ikimenyetso cya 0.1% cya kristalo violet (aside acetic glacial)
- 2. Kumenya aside amine zifunguye
Ingero zarumishijwe kuri dogere selisiyusi 80 mu gihe cy'isaha imwe.
Shyira icyitegererezo mu kintu cyumye kugira ngo gikonje mu buryo busanzwe kugeza ku bushyuhe bw'icyumba cyangwa gikonje kugeza ku bushyuhe bushoboka.Pima hafi garama 0.1 z'icyitegererezo (kimeze neza kugeza kuri garama 0.001) mu icupa ryumye rya mL 250.Komeza vuba intambwe ikurikiraho kugira ngo wirinde ko icyitegererezo cyakwinjirira ubushuhe bwo mu kirereOngeraho mililitiro 25 za aside asetiki y'urubura hanyuma uvange neza mu gihe kitarenze iminota 5.Ongeraho ibitonyanga 2 by'ikimenyetso cya crystal violetTitrate hamwe na 0.0500 mol / L (± 0.001) igisubizo gisanzwe cya titration cya aside perchloric kugeza igihe igisubizo gihindutse kuva ku ibara ry'umuhengeri kugera ku iherezo.
Andika ingano y'umuti usanzwe wakoreshejwe.
- Kora ikizamini cy'ubusa icyarimwe.
- 3. Kubara n'ibisubizo
- Ingano ya aside amine X iri muri reagent igaragazwa nk'igice cy'uburemere (%) kandi ibarwa hakurikijwe formula: X = C × (V1-V0) × 0.1445/M × 100%, muri formula ya tne:
- C - Ingano y'umuti usanzwe wa aside perchloric muri moles kuri litiro (mol/L)
- V1 - Ingano ikoreshwa mu gupima ingero hakoreshejwe umuti usanzwe wa aside perchloric, muri mililitiro (mL).
- Vo - Ingano ikoreshwa mu gupima ubusa hamwe n'umuti usanzwe wa aside perchloric, muri mililitiro (mL);
M - Uburemere bw'icyitegererezo, muri garama (g).
| 0.1445: Uburemere bw'impuzandengo ya aside amine bungana na mL 1.00 z'umuti usanzwe wa aside perchloric [c (HClO4) = 1.000 mol / L]. | 4.2.3 Umuti usanzwe wa Cerium sulfate: igipimo c [Ce (SO4) 2] = 0.1 mol/L, wateguwe hakurikijwe GB/T601. | |
| Kwemeza amahame ngenderwaho: Q/70920556 71-2024 | 1. Ihame ryo guhitamo (urugero rwa Fe) | Ibyuma bya aside amino bifite ubushobozi buke bwo gushonga muri ethanol idafite amazi kandi iyoni z’icyuma zishongeshwa muri ethanol idafite amazi, itandukaniro mu gushonga hagati y’ibyo bibiri muri ethanol idafite amazi ryakoreshejwe kugira ngo hamenyekane igipimo cya chelation cy’ibice bya aside amino icyuma. |
| Muri formula: V1 - ingano y'umuti usanzwe wa cerium sulfate ukoreshwa mu gupima umuti, mL; | Ethanol idakoresha amazi; ibisigaye ni kimwe n'ingingo ya 4.5.2 muri GB/T 27983-2011. | 3. Intambwe zo gusesengura |
| Kora igerageza ribiri icyarimwe. Pima garama 0.1 z'icyitegererezo cyumye kuri dogere 103±2℃ mu gihe cy'isaha 1, neza kuri garama 0.0001, ongeramo garama 100 za etanoli idafite amazi kugira ngo ishonge, yungurura, yungurura ibisigazwa byogejwe na garama 100 za etanoli idafite amazi nibura inshuro eshatu, hanyuma ushyire ibisigazwa mu icupa rya garama 250, ongeramo garama 10 z'umuti wa aside sulfure nk'uko bivugwa mu ngingo ya 4.5.3 muri GB/T27983-2011, hanyuma ukore ibi bikurikira nk'uko bivugwa mu ngingo ya 4.5.3 "Shusha kugira ngo ishonge hanyuma uyireke ikonje" muri GB/T27983-2011. Kora ikizamini cy'ubusa icyarimwe. | 4. Kumenya ingano y'icyuma cyose | 4.1 Ihame ryo kugena ni kimwe n'ingingo ya 4.4.1 muri GB/T 21996-2008. |
4.2. Ibikoresho bitanga umusaruro n'ibisubizo
| 4.2.1 Aside ivanze: Shyira mililitiro 150 za aside sulfurike na mililitiro 150 za aside phosphorike muri mililitiro 700 z'amazi hanyuma uvange neza. | 4.2.2 Umuti w'ikimenyetso cya Sodium diphenylamine sulfonate: 5g/L, wateguwe hakurikijwe GB/T603. | 4.2.3 Umuti usanzwe wa Cerium sulfate: igipimo c [Ce (SO4) 2] = 0.1 mol/L, wateguwe hakurikijwe GB/T601. | |
| 4.3 Intambwe zo gusesengura | Kora igerageza ribiri icyarimwe. Pima garama 0.1 z'icyitegererezo, gihuye na garama 020001, shyira mu icupa rya mililitiro 250, ongeramo mililitiro 10 z'aside ivanze, nyuma yo gushonga, ongeramo mililitiro 30 z'amazi n'ibitonyanga 4 bya sodium dianiline sulfonate, hanyuma ukore ibi bikurikira nk'uko bivugwa mu ngingo ya 4.4.2 muri GB/T21996-2008. Kora ikizamini cy'ubusa icyarimwe. | 4.4 Igaragazwa ry'ibyavuye mu bushakashatsi | Igiteranyo cy'icyuma X1 cy'ibice by'icyuma bya aside amine mu buryo bw'igice cy'icyuma, agaciro kagaragazwa muri %, kabazwe hakurikijwe formula (1): |
| X1 = (V-V0) × C × M × 10-3 × 100 | V0 - umuti usanzwe wa cerium sulfate ukoreshwa mu gupima umuti udafite ikintu, mL; | V0 - umuti usanzwe wa cerium sulfate ukoreshwa mu gupima umuti udafite ikintu, mL; | C - Igipimo nyacyo cy'umuti usanzwe wa cerium sulfate, mol/L5. Kubara ingano y'icyuma muri chelateIngano y'icyuma X2 muri chelate hakurikijwe igice cy'uburemere bw'icyuma, agaciro kagaragazwa muri %, kabazwe hakurikijwe formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Muri formula: V1 - ingano y'umuti usanzwe wa cerium sulfate ukoreshwa mu gupima umuti, mL; | V2 - umuti usanzwe wa cerium sulfate ukoreshwa mu gupima umuti udafite ikintu, mL;nom1 - Uburemere bw'icyitegererezo, g. Fata impuzandengo y'imibare y'ibyavuye mu gupima parallel nk'ibisubizo by'igenamigambi, kandi itandukaniro risesuye ry'ibyavuye mu gupima parallel ntirirenze 0.3%. | 0.05585 - uburemere bw'icyuma cya ferrous cyagaragaye muri garama zingana na 1.00 mL y'umuti usanzwe wa cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1 - Uburemere bw'icyitegererezo, g. Fata impuzandengo y'imibare y'ibyavuye mu gupima parallel nk'ibisubizo by'igenamigambi, kandi itandukaniro risesuye ry'ibyavuye mu gupima parallel ntirirenze 0.3%. | 6. Kubara igipimo cya chelationIgipimo cya Chelation X3, agaciro kagaragajwe muri %, X3 = X2/X1 × 100Umugereka C: Uburyo bwo kumenya igipimo cya Zinpro cyo gupima ingano y'amaraso |
Kwemeza ibipimo ngenderwaho: Q/320205 KAVNO7-2016
1. Ibikoresho bitanga umusaruro n'ibikoresho
a) Aside acetike y'ibara rya glacial: isukuye mu buryo bw'isesengura; b) Aside ya perchloric: 0.0500mol/L; c) Ikimenyetso: Ikimenyetso cya 0.1% cya crystal violet (aside acetike y'ibara rya glacial)
2. Kumenya aside amine zifunguye
2.1 Ingero zarumishijwe kuri dogere selisiyusi 80 mu gihe cy'isaha imwe.
2.2 Shyira icyitegererezo mu kintu cyumye kugira ngo gikonje mu buryo busanzwe kugeza ku bushyuhe bw'icyumba cyangwa gikonje kugeza ku bushyuhe bushoboka.
2.3 Pima hafi garama 0.1 z'icyitegererezo (kimeze neza kugeza kuri garama 0.001) mu icupa ryumye rya mL 250
2.4 Komeza vuba intambwe ikurikira kugira ngo wirinde ko icyitegererezo cyakwinjirira ubushuhe buri mu kirere.
2.5 Ongeramo 25ml ya aside asetiki y'urubura hanyuma uvange neza mu gihe kitarenze iminota 5.
2.6 Ongeraho ibitonyanga 2 by'ikimenyetso cya crystal violet.
2.7 Titrate hamwe na 0.0500mol/L (±0.001) umuti usanzwe wa titration wa aside perchloric kugeza igihe umuti uhindutse kuva ku ibara ry'umuhengeri ukajya ku ibara ry'icyatsi mu gihe cy'iminota 15 nta guhindura ibara nk'aho ari iherezo.
2.8 Andika ingano y'umuti usanzwe wakoreshejwe.
2.9 Kora ikizamini cy'ubusa icyarimwe.
- 3. Kubara n'ibisubizo
- Igikatalani
- Physicochemical parameters
V1 - Ingano ikoreshwa mu gupima ingero hakoreshejwe umuti usanzwe wa aside perchloric, muri mililitiro (mL).
Vo - Ingano ikoreshwa mu gupima ubusa hamwe n'umuti usanzwe wa aside perchloric, muri mililitiro (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Aderesi: No.147 Qingpu Road, Umujyi wa Shouan, Akarere ka Pujiang, Umujyi wa Chengdu, Intara ya Sichuan, mu Bushinwa
Terefone: 86-18880477902
Ibicuruzwa
Imyunyu ngugu idakomoka ku bimera
- Imyunyu ngugu y'ibinyabuzima
- Igiswahili
- Serivisi yihariye
- Amasano yihuse
Umwirondoro w'ikigo
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Igigujarati | Kanda kugira ngo ubaze | © Uburenganzira bwose burasubitswe - 2010-2025 : Uburenganzira bwose burasubitswe. | Ikarita y'urubuga GUSHAKA KW'ISHUSHO RY'ICYUBAHIRO Terefone |
| Terefone | 86-18880477902 | Ikijava | Imeri |
| 8618880477902 | Igishinwa | Igifaransa | |
| Bird | Igishinwa | Igifaransa | Ikidage Icyesipanyoli |
| Aquatic animals | Ikiyapani | Igikoreya | Icyarabu Ikigereki |
| Igiturukiya | Igitaliyani | ||
| Ruminant animal g/head day | January 0.75 | Ikinyandoneziya Ikinyafurika Igisuwede |
Igipolonye
- Ikibasiki
- Igikatalani
- Physicochemical parameters
Igihindi
Ikilawoniya
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Igishona
Ikinyabulgariya
- Igicebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Igikorowasiya
Ikiholandi
| Application object | Ikinyaurudu Ikiviyetinamu | Content in full-value feed (mg/kg) | Efficacy |
| Igigujarati | Haiti | Igihawusa | Ikinyarwanda Igihmong Igihongiriya |
| Piglets and fattening pigs | Igbo | Ikijava | Igikannada Igikhmer Igikurdi |
| Ikigizi | Ikilatini | ||
| Bird | 300～400 | 45～60 | Ikimasedoniya Ikimalayimeya Ikimalayalamu |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Ikinyanoruveje
- Igipashito
- Appearance: brownish-yellow granules
- Physicochemical parameters
Igiseribe
Igisuto
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Igishona
Igisindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Igiswahili
Igitajiki
Igitamili
Igitelugu
Igitayilandi
| Application object | Ikinyaurudu Ikiviyetinamu | Content in full-value feed (mg/kg) | Efficacy |
| Ikiyidishi | Ikiyoruba | Ikizulu | Ikinyarwanda Ikioriya Abanyaturuki |
| Ikiyugur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
